
A easy methodology for ex vivo honey bee cell tradition able to in vitro gene expression evaluation
A novel 3D tradition mannequin recapitulates major FL B cell options and promotes their survival
- Utilizing a microfluidic methodology primarily based on cell encapsulation inside permeable, elastic, and hole alginate microspheres, we developed a brand new tunable 3D-model incorporating lymphoma B cells, extracellular matrix (ECM), and/or tonsil stromal cells (TSC).
- We revealed that beneath 3D confinement lymphoma B cells had been in a position to kind cohesive spheroids ensuing from overexpression of ECM elements. Furthermore, lymphoma B cells and TSC dynamically shaped self-organized 3D spheroids favoring spheroid progress. 3D tradition induced resistance to classical chemotherapeutic agent doxorubicin, however to not BCL2 inhibitor ABT-199, figuring out this method as a related in vitro mannequin to evaluate the exercise of therapeutic brokers in B-NHL.
- RNAseq evaluation highlighted the synergy of 3D, ECM, and TSC in upregulating related pathways in malignant B cells in vitro than these overexpressed in major lymphoma cells in situ. Lastly, our 3D mannequin together with ECM and TSC allowed long-term in vitro survival of major follicular lymphoma B cells.
- In conclusion, we suggest a brand new excessive throughput 3D mannequin mimicking lymphoma tumor area of interest and making it potential to check the dynamic relationship between lymphoma B cells and their microenvironment and to display new anti-cancer medication.
Tradition of rat mesenchymal stem cells on PHBV-PCL scaffolds: evaluation of conditioned tradition medium by FT-Raman spectroscopy
Characterization of cell cycle and apoptosis in CHO cell tradition utilizing circulation cytometry for bioprocess monitoring
Semaphorin 6A (SEMA6A) Antibody |
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abx031679-80l | Abbexa | 80 µl | EUR 286 |
Semaphorin 6A (SEMA6A) Antibody |
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abx031680-400ul | Abbexa | 400 ul | EUR 523 |
Semaphorin 6A (SEMA6A) Antibody |
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abx031680-80l | Abbexa | 80 µl | EUR 286 |
Semaphorin 6A (SEMA6A) Antibody |
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20-abx212108 | Abbexa |
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Semaphorin 6A (SEMA6A) Antibody |
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20-abx211651 | Abbexa |
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Mouse Semaphorin- 6A, Sema6a ELISA KIT |
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ELI-52928m | Lifescience Market | 96 Tests | EUR 865 |
Human Semaphorin 6A(SEMA6A)ELISA Kit |
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GA-E1104HM-48T | GenAsia Biotech | 48T | EUR 289 |
Human Semaphorin 6A(SEMA6A)ELISA Kit |
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GA-E1104HM-96T | GenAsia Biotech | 96T | EUR 466 |
Human Semaphorin 6A (SEMA6A)ELISA Kit |
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201-12-1088 | SunredBio | 96 tests | EUR 440 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Semaphorin 6A(SEMA6A)ELISA Kit |
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QY-E00788 | Qayee Biotechnology | 96T | EUR 361 |
Human Semaphorin- 6A, SEMA6A ELISA KIT |
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ELI-52440h | Lifescience Market | 96 Tests | EUR 824 |
Mouse anti-SAH 6a |
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MA00306-50 | Arthus | 50µg | EUR 348 |
Description: Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 844-2 |
Rabbit Polyclonal antibody Anti-CRBN |
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Anti-CRBN | ImmunoStep | 50 µg | EUR 349 |
Anti-Rab 6A Antibody |
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A02911 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal Antibody for Rab 6A Antibody (RAB6A) detection.tested for WB in Human, Mouse, Rat. |
Anti-Rab 6A antibody |
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STJ95302 | St John's Laboratory | 200 µl | EUR 197 |
Description: Rabbit polyclonal to Rab 6A. |
anti- Cytokeratin 6A antibody |
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FNab02215 | FN Test | 100µg | EUR 548.75 |
Description: Antibody raised against Cytokeratin 6A |
Anti-Cytokeratin 6A antibody |
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PAab02215 | Lifescience Market | 100 ug | EUR 386 |
anti-Anti-Semaphorin 5A |
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YF-PA25252 | Abfrontier | 50 ul | EUR 334 |
Description: Mouse polyclonal to Anti-Semaphorin 5A |
anti-Semaphorin 3D |
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YF-PA22776 | Abfrontier | 50 ug | EUR 363 |
Description: Mouse polyclonal to Semaphorin 3D |
anti-Semaphorin 3D |
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YF-PA22777 | Abfrontier | 100 ug | EUR 403 |
Description: Rabbit polyclonal to Semaphorin 3D |
anti-Semaphorin 3B |
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YF-PA25020 | Abfrontier | 50 ul | EUR 334 |
Description: Mouse polyclonal to Semaphorin 3B |
anti-Semaphorin 7a |
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YF-PA25109 | Abfrontier | 50 ul | EUR 334 |
Description: Mouse polyclonal to Semaphorin 7a |
anti-Semaphorin 3A |
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YF-PA25559 | Abfrontier | 50 ul | EUR 334 |
Description: Mouse polyclonal to Semaphorin 3A |
anti-Semaphorin 3B |
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YF-PA15485 | Abfrontier | 100 ug | EUR 403 |
Description: Rabbit polyclonal to Semaphorin 3B |
anti- Cytokeratin 6A-specific antibody |
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FNab02216 | FN Test | 100µg | EUR 548.75 |
Description: Antibody raised against Cytokeratin 6A-specific |
Anti-Cytokeratin 6A-specific antibody |
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PAab02216 | Lifescience Market | 100 ug | EUR 386 |
Anti Mouse Semaphorin 3B Polyclonal Antibody |
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KR100 | Sceti | EUR 649 | |
Description: The Anti Mouse Semaphorin 3B Polyclonal Antibody is available in Europe and for worldwide shipping via Gentaur. |
Anti-Semaphorin 5A antibody |
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STJ70970 | St John's Laboratory | 100 µg | EUR 359 |
Anti-Semaphorin 7a (3D3) |
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YF-MA16372 | Abfrontier | 100 ug | EUR 363 |
Description: Mouse monoclonal to Semaphorin 7a |
Anti-Semaphorin 7a (1G1) |
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YF-MA16373 | Abfrontier | 100 ug | EUR 363 |
Description: Mouse monoclonal to Semaphorin 7a |