
A easy methodology for ex vivo honey bee cell tradition able to in vitro gene expression evaluation
A novel 3D tradition mannequin recapitulates major FL B cell options and promotes their survival
- Utilizing a microfluidic methodology primarily based on cell encapsulation inside permeable, elastic, and hole alginate microspheres, we developed a brand new tunable 3D-model incorporating lymphoma B cells, extracellular matrix (ECM), and/or tonsil stromal cells (TSC).
- We revealed that beneath 3D confinement lymphoma B cells had been in a position to kind cohesive spheroids ensuing from overexpression of ECM elements. Furthermore, lymphoma B cells and TSC dynamically shaped self-organized 3D spheroids favoring spheroid progress. 3D tradition induced resistance to classical chemotherapeutic agent doxorubicin, however to not BCL2 inhibitor ABT-199, figuring out this method as a related in vitro mannequin to evaluate the exercise of therapeutic brokers in B-NHL.
- RNAseq evaluation highlighted the synergy of 3D, ECM, and TSC in upregulating related pathways in malignant B cells in vitro than these overexpressed in major lymphoma cells in situ. Lastly, our 3D mannequin together with ECM and TSC allowed long-term in vitro survival of major follicular lymphoma B cells.
- In conclusion, we suggest a brand new excessive throughput 3D mannequin mimicking lymphoma tumor area of interest and making it potential to check the dynamic relationship between lymphoma B cells and their microenvironment and to display new anti-cancer medication.
Tradition of rat mesenchymal stem cells on PHBV-PCL scaffolds: evaluation of conditioned tradition medium by FT-Raman spectroscopy
Characterization of cell cycle and apoptosis in CHO cell tradition utilizing circulation cytometry for bioprocess monitoring
Semaphorin 6A (SEMA6A) Antibody |
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abx031680-400ul | Abbexa | 400 ul | EUR 627.6 |
Semaphorin 6A (SEMA6A) Antibody |
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abx031680-80l | Abbexa | 80 µl | EUR 343.2 |
Semaphorin 6A (SEMA6A) Antibody |
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20-abx302320 | Abbexa |
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Semaphorin 6A (SEMA6A) Antibody |
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20-abx211651 | Abbexa |
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Semaphorin 6A (SEMA6A) Antibody |
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20-abx212108 | Abbexa |
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Semaphorin 6A Antibody / SEMA6A |
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RQ5921 | NSJ Bioreagents | 100 ug | EUR 419 |
Mouse Semaphorin- 6A, Sema6a ELISA KIT |
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ELI-52928m | Lifescience Market | 96 Tests | EUR 1038 |
Human Semaphorin 6A (SEMA6A)ELISA Kit |
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201-12-1088 | SunredBio | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Semaphorin- 6A, SEMA6A ELISA KIT |
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ELI-52440h | Lifescience Market | 96 Tests | EUR 988.8 |
Human Semaphorin 6A(SEMA6A)ELISA Kit |
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GA-E1104HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human Semaphorin 6A(SEMA6A)ELISA Kit |
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GA-E1104HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Human Semaphorin 6A(SEMA6A)ELISA Kit |
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QY-E00788 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Semaphorin 6A (SEMA6A)ELISA Kit |
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YLA2513HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human Semaphorin 6A (SEMA6A)ELISA Kit |
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YLA2513HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
Mouse anti-SAH 6a |
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MA00306-50 | Arthus | 50µg | EUR 417.6 |
Description: Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 844-2 |
Rabbit Polyclonal antibody Anti-CRBN |
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Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
Anti-Rab 6A Antibody |
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A02911 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for Rab 6A Antibody (RAB6A) detection.tested for WB in Human, Mouse, Rat. |
Anti-Cytokeratin 6A antibody |
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PAab02215 | Lifescience Market | 100 ug | EUR 463.2 |
anti- Cytokeratin 6A antibody |
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FNab02215 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against Cytokeratin 6A |
Anti-Rab 6A antibody |
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STJ95302 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Rab 6A. |
anti-Semaphorin 3D |
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YF-PA22776 | Abfrontier | 50 ug | EUR 435.6 |
Description: Mouse polyclonal to Semaphorin 3D |
anti-Semaphorin 3D |
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YF-PA22777 | Abfrontier | 100 ug | EUR 483.6 |
Description: Rabbit polyclonal to Semaphorin 3D |
anti-Semaphorin 3B |
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YF-PA25020 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to Semaphorin 3B |
anti-Semaphorin 7a |
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YF-PA25109 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to Semaphorin 7a |
anti-Semaphorin 3A |
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YF-PA25559 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to Semaphorin 3A |
anti-Semaphorin 3B |
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YF-PA15485 | Abfrontier | 100 ug | EUR 483.6 |
Description: Rabbit polyclonal to Semaphorin 3B |
anti-Anti-Semaphorin 5A |
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YF-PA25252 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to Anti-Semaphorin 5A |
Keratin 6A Antibody / Cytokeratin 6A |
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V8960-100UG | NSJ Bioreagents | 100ug | EUR 499 |
Description: Cytokeratin 6 is an epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. This protein always paired with the type-I keratin K16. Cytokeratin 6 have been identified in epidermis only in samples from plantar glabrous skin while in hairy skin this keratin appeared to be absent in interfollicular epidermis but were clearly present in hair follicle outer root sheath, and this protein also consistently expressed in non-keratinizing stratified squamous epithelia. Among tumors, cytokeratin 6 is typically and strongly expressed in squamous cell carcinomas of different sites, preferentially in inner, maturing layers of the tumor cell nests. Cytokeratin 6 is always used with cytokeratin 5 and is a useful aid for classification of epithelioid mesotheliomas. |
Keratin 6A Antibody / Cytokeratin 6A |
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V8960-20UG | NSJ Bioreagents | 20ug | EUR 219 |
Description: Cytokeratin 6 is an epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. This protein always paired with the type-I keratin K16. Cytokeratin 6 have been identified in epidermis only in samples from plantar glabrous skin while in hairy skin this keratin appeared to be absent in interfollicular epidermis but were clearly present in hair follicle outer root sheath, and this protein also consistently expressed in non-keratinizing stratified squamous epithelia. Among tumors, cytokeratin 6 is typically and strongly expressed in squamous cell carcinomas of different sites, preferentially in inner, maturing layers of the tumor cell nests. Cytokeratin 6 is always used with cytokeratin 5 and is a useful aid for classification of epithelioid mesotheliomas. |
Keratin 6A Antibody / Cytokeratin 6A |
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V8960SAF-100UG | NSJ Bioreagents | 100ug | EUR 499 |
Description: Cytokeratin 6 is an epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. This protein always paired with the type-I keratin K16. Cytokeratin 6 have been identified in epidermis only in samples from plantar glabrous skin while in hairy skin this keratin appeared to be absent in interfollicular epidermis but were clearly present in hair follicle outer root sheath, and this protein also consistently expressed in non-keratinizing stratified squamous epithelia. Among tumors, cytokeratin 6 is typically and strongly expressed in squamous cell carcinomas of different sites, preferentially in inner, maturing layers of the tumor cell nests. Cytokeratin 6 is always used with cytokeratin 5 and is a useful aid for classification of epithelioid mesotheliomas. |