The ectoparasite protozoan Amyloodinium ocellatum (AO) is the causative agent of amyloodiniosis in European seabass (ESB, Dicentrarchus labrax). There’s a lack of expertise about fundamental molecular immune response mechanisms of ESB throughout AO infestation. Subsequently, to check gene expression between experimental AO-infested ESB tissues and uninfested ESB tissues (gills and head kidney) RNA-seq was adopted. The RNA-seq revealed a number of differentially expressed genes (DEG), particularly 679 upregulated genes and 360 downregulated genes within the gills, and 206 upregulated genes and 170 downregulated genes in head kidney.
In gills, genes associated to the immune system (perforin, CC1) and protein binding have been upregulated. A number of genes concerned in IFN associated pathways have been upregulated within the head kidney. Subsequently, to validate the DEG from amyloodiniosis, 26 ESB (imply weight 14 g) per tank in triplicate have been tub challenged for two h with AO (3.5 × 106/tank; 70 dinospores/mL) below managed circumstances (26-28 °C and 34‰ salinity). As a management group (non-infested), 26 ESB per tank in triplicate have been additionally used. Adjustments within the expression of innate immune genes in gills and head kidney at 2, 3, 5, 7 and 23 dpi have been analysed utilizing real-time PCR.
The outcomes indicated that the expression of cytokines (CC1, IL-8) and antimicrobial peptide (Hep) have been strongly stimulated and reached a peak at 5 dpi within the early infestation stage, adopted by a gradual discount within the restoration stage (23 dpi). Noticeably, the immunoglobulin (IgM) expression was greater at 23 dpi in comparison with 7 dpi. Moreover, in-situ hybridization confirmed constructive alerts of CC1 mRNA in AO infested gills in comparison with the management group. Altogether, chemokines have been concerned within the immune course of below AO infestation and this proof permits a greater understanding of the immune response in European seabass throughout amyloodiniosis.
Incidence of Clinically Vital Aniseikonia Following Encircling Scleral Buckle Surgical procedure: An Analysis of Refractive and Axial Size Adjustments Requiring Intervention
To judge the incidence of symptomatic anisometropia and aniseikonia requiring intervention following surgical procedure with mixed pars plana vitrectomy (PPV) and broad 276 model encircling scleral buckle (ESB) for the restore of rhegmatogenous retinal detachments (RRD) and to report axial size (AL) and keratometry modifications, a retrospective evaluate of consecutive RRD sufferers handled with mixed PPV and ESB between June 2016 till September 2019 was carried out. All sufferers with symptomatic optically induced aniseikonia requiring further interventions or surgical procedures together with clear lens exchanges, secondary intraocular lens implants or contact lenses have been documented. Keratometry and AL measurements have been recorded for every eye and modifications calculated.
In complete, 100 sufferers underwent mixed PPV, ESB and endotamponade with imply age of 59.47 years. AL was considerably elevated to 26.54 mm, with a imply change of 1.15 mm. Imply corneal astigmatism elevated by -0.95 D in management eyes preoperatively and -1.33 postoperatively . Over half of phakic sufferers (39/61; 64%) developed a visually vital cataract, subsequently present process surgical procedure. Six of 100 sufferers developed symptomatic anisometropia with aniseikonia postoperatively (6%). 4 proceeded with clear lens change regardless of absence of visually vital cataract (4%).
Two of those initially trialled contact lenses. One was illiberal, whereas the opposite determined to proceed with clear lens change for comfort. Just one affected person (1%), being pseudophakic in each eyes, had persistent anisometropia/aniseikonia. AL and keratometry modifications induced by encirclement with broad strong silicone rubber buckles are acceptable and much like these reported beforehand utilizing slender encircling elements, being unlikely to induce troublesome symptomatic anisometropia/aniseikonia. Many sufferers are phakic and develop visually vital cataracts, permitting correction of modifications induced with the goal of visible restoration. A minority require extra extended strategies of visible rehabilitation, comparable to contact lens put on or clear lens exchanges. Warning and acceptable consent must be made in sufferers which can be pseudophakic in each eyes at presentation.
Prevalence and correlates of maternal early stimulation behaviors throughout being pregnant in northern Ghana: a cross-sectional survey
Bupivacaine hydrochloride |
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GP3506-100G | Glentham Life Sciences | 100 g | EUR 313.2 |
Bupivacaine hydrochloride |
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GP3506-25G | Glentham Life Sciences | 25 g | EUR 141.6 |
Bupivacaine hydrochloride |
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GP3506-5G | Glentham Life Sciences | 5 g | EUR 74.4 |
Bupivacaine hydrochloride |
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GP3506-100 | Glentham Life Sciences | 100 | EUR 229.3 |
Bupivacaine hydrochloride |
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GP3506-25 | Glentham Life Sciences | 25 | EUR 87.1 |
Bupivacaine hydrochloride |
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GP3506-5 | Glentham Life Sciences | 5 | EUR 31.7 |
Desbutyl Lumefantrine D9 |
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HY-12781S | MedChemExpress | 10mg | EUR 2456.4 |
L-Bupivacaine |
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GP4736-100MG | Glentham Life Sciences | 100 mg | EUR 180 |
L-Bupivacaine |
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GP4736-25MG | Glentham Life Sciences | 25 mg | EUR 103.2 |
L-Bupivacaine |
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GP4736-100 | Glentham Life Sciences | 100 | EUR 118.7 |
L-Bupivacaine |
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GP4736-25 | Glentham Life Sciences | 25 | EUR 55.4 |
Bupirimate |
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S-4272 | Scientific Laboratory Supplies | 1ML | EUR 92.4 |
(Des-Bromo)-Neuropeptide B (1-23) (human) |
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H-5906.0001 | Bachem | 1.0mg | EUR 1009.2 |
Description: Sum Formula: C107H162N30O30; CAS# [434897-64-0] net |
(Des-Bromo)-Neuropeptide B (1-23) (human) |
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H-5906.0005 | Bachem | 5.0mg | EUR 3924 |
Description: Sum Formula: C107H162N30O30; CAS# [434897-64-0] net |
Desert Hedgehog/Dhh N-Terminus |
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MO15135 | Neuromics | 500 ug | EUR 732 |
Recombinant Human Desmin (N-6His) |
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CE67-10ug | Novoprotein | 10ug | EUR 242.4 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Recombinant Human Desmin (N-6His) |
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CE67-1mg | Novoprotein | 1mg | EUR 2739.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Recombinant Human Desmin (N-6His) |
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CE67-500ug | Novoprotein | 500ug | EUR 1935.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Recombinant Human Desmin (N-6His) |
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CE67-50ug | Novoprotein | 50ug | EUR 595.2 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
DES Protein Vector (Rat) (pPB-N-His) |
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PV263871 | ABM | 500 ng | EUR 723.6 |
DES Protein Vector (Human) (pPB-N-His) |
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PV012234 | ABM | 500 ng | EUR 394.8 |
DES Protein Vector (Mouse) (pPB-N-His) |
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PV171755 | ABM | 500 ng | EUR 723.6 |
BUP-1 Recombinant Protein |
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91-190 | ProSci | 0.05 mg | EUR 852.9 |
Description: beta -Ureidopropionase is a cytoplasmic protein which belongs to the CN hydrolase family of BUP subfamily. beta -Ureidopropionase binds one zinc ion per subunit, catalyzes the last step in the pyrimidine degradation pathway. beta -Ureidopropionase can convert N-carbamyl-beta-aminoisobutyric acid and N-carbamyl-beta-alanine to beta-aminoisobutyric acid and beta-alanine, ammonia and carbon dioxide, respectively. The pyrimidine bases uracil and thymine are degraded via the consecutive action of dihydropyrimidine dehydrogenase (DHPDH), dihydropyrimidinase (DHP) and beta-ureidopropionase (UP) to beta-alanine and beta aminoisobutyric acid, respectively. Defects in beta -Ureidopropionase are the cause of beta -Ureidopropionase deficiency that is characterized by muscular hypotonia, dystonic movements, scoliosis, microcephaly and severe developmental delay. |
N, N, N′, N′-Tetramethylethylenediamine |
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abx082512-100ml | Abbexa | 100 ml | EUR 243.6 |
N, N, N′, N′-Tetramethylethylenediamine |
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abx082540-100ml | Abbexa | 100 ml | EUR 243.6 |
N,N,N',N'-Tetramethylmalonamide |
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abx188802-500g | Abbexa | 500 g | EUR 2448 |
N,N,N',N'-Tetramethylethylendiamine |
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GE3482-100ML | Glentham Life Sciences | 100 ml | EUR 133.2 |
N,N,N',N'-Tetramethylethylendiamine |
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GE3482-25ML | Glentham Life Sciences | 25 ml | EUR 69.6 |
N,N,N'',N''-Tetramethylethylendiamine |
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GE3482-100 | Glentham Life Sciences | 100 | EUR 80.4 |
N,N,N'',N''-Tetramethylethylendiamine |
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GE3482-25 | Glentham Life Sciences | 25 | EUR 27.8 |
Buprenorphine (BUP)-BSA Antigen (HM376 or HM377) |
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VAng-Cr3660-1mg | Creative Biolabs | 1 mg | EUR 720 |
Description: Buprenorphine (BUP)-BSA Antigen, matched pairs: HM376 or HM377 (detection); Used for ELISA, Lateral Flow. |
Buprenorphine (BUP)-BSA Antigen (P02-99-41M-P, P02-99-42M-P) |
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VAng-Cr3662-1mg | Creative Biolabs | 1 mg | EUR 885.6 |
Description: Buprenorphine (BUP)-BSA Antigen, matched pairs: P02-99-41M-P, P02-99-42M-P; Used for ELISA, Lateral Flow. |
8-Hydroxy-N,N,N'',N'',N'''',N''''-hexamethyl-pyrene-1,3,6-trisulfonamide |
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GW8063-10 | Glentham Life Sciences | 10 | EUR 292.5 |
8-Hydroxy-N,N,N'',N'',N'''',N''''-hexamethyl-pyrene-1,3,6-trisulfonamide |
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GW8063-50 | Glentham Life Sciences | 50 | EUR 1031.5 |
Buprenorphine (BUP)-BSA Antigen (HM376 or HM377) (Hapten:Protein=25:1) |
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VAng-Cr3661-1mg | Creative Biolabs | 1 mg | EUR 555.6 |
Description: Buprenorphine (BUP)-BSA Antigen, matched pairs: HM376 or HM377 (detection); Hapten:Protein=25:1; Used for ELISA, Lateral Flow. |
N,N-Dimethyldecylamine N-oxide |
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GL4652-5G | Glentham Life Sciences | 5 g | EUR 495.6 |
N,N-Dimethyldecylamine N-oxide |
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GL4652-5 | Glentham Life Sciences | 5 | EUR 379.6 |
N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine |
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GK2289-100MG | Glentham Life Sciences | 100 mg | EUR 132 |
N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine |
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GK2289-250MG | Glentham Life Sciences | 250 mg | EUR 217.2 |
N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine |
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GK2289-50MG | Glentham Life Sciences | 50 mg | EUR 92.4 |
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride |
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GK6593-100G | Glentham Life Sciences | 100 g | EUR 351.6 |
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride |
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GK6593-25G | Glentham Life Sciences | 25 g | EUR 141.6 |
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride |
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GK6593-50G | Glentham Life Sciences | 50 g | EUR 217.2 |
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride |
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GK6593-5G | Glentham Life Sciences | 5 g | EUR 68.4 |
N,N,N'',N''-Tetramethyl-p-phenylenediamine dihydrochloride |
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GK6593-100 | Glentham Life Sciences | 100 | EUR 261 |
N,N,N'',N''-Tetramethyl-p-phenylenediamine dihydrochloride |
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GK6593-25 | Glentham Life Sciences | 25 | EUR 87.1 |
N,N,N'',N''-Tetramethyl-p-phenylenediamine dihydrochloride |
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GK6593-5 | Glentham Life Sciences | 5 | EUR 26.9 |
N,N,N'',N''-Tetramethyl-p-phenylenediamine dihydrochloride |
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GK6593-50 | Glentham Life Sciences | 50 | EUR 150.4 |
N,N,N'',N''-Tetrakis(2-pyridylmethyl)ethylenediamine |
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GK2289-100 | Glentham Life Sciences | 100 | EUR 79.1 |
N,N,N'',N''-Tetrakis(2-pyridylmethyl)ethylenediamine |
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GK2289-250 | Glentham Life Sciences | 250 | EUR 150.4 |
N,N,N'',N''-Tetrakis(2-pyridylmethyl)ethylenediamine |
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GK2289-50 | Glentham Life Sciences | 50 | EUR 46.8 |
N,N‐DIISOPROPYLFORMAMIDE |
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504018 | Survival Technologies | each | Ask for price |
N,N‐DIISOPROPYLETHYLAMINE |
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704006 | Survival Technologies | each | Ask for price |
N,N-Dimethylsphingosine |
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20-abx076692 | Abbexa |
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N, N-Dimethylformamide |
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abx082329-100ml | Abbexa | 100 ml | EUR 226.8 |
N, N-Dimethylformamide |
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abx082489-100ml | Abbexa | 100 ml | EUR 210 |
N, N′-Methylenebisacrylamide |
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20-abx082510 | Abbexa |
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N, N′-Methylenebisacrylamide |
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20-abx082531 | Abbexa |
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N,N-Dimethylsphingosine |
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B7694-10 | ApexBio | 10 mg | EUR 236.4 |
N,N-Dimethylsphingosine |
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B7694-25 | ApexBio | 25 mg | EUR 480 |
N,N-Dimethylsphingosine |
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B7694-5 | ApexBio | 5 mg | EUR 153.6 |
N,N-Dimethylsphingosine |
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B7694-50 | ApexBio | 50 mg | EUR 807.6 |
N,N'-Dicyclohexylurea |
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C3458-10000 | ApexBio | 10 g | EUR 85.2 |
N,N'-Dicyclohexylurea |
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C3458-25000 | ApexBio | 25 g | EUR 103.2 |
N,N-Dimethylnicotinamide |
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20-abx186517 | Abbexa |
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N,N'-Methylenebisacrylamide |
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abx188804-1kg | Abbexa | 1 kg | EUR 543.6 |
N,N-Diethylacetamide |
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20-abx180831 | Abbexa |
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N,N'-diisopropylethylenediamine |
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20-abx181328 | Abbexa |
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N,N-Dibenzylformamide |
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20-abx182586 | Abbexa |
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N,N'-Dicyclohexylcarbodiimide |
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abx184247-1kg | Abbexa | 1 kg | EUR 360 |
N,N'-Ethylenethiourea |
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GK2739-100G | Glentham Life Sciences | 100 g | EUR 62.4 |
N,N'-Ethylenethiourea |
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GK2739-1KG | Glentham Life Sciences | 1 kg | EUR 141.6 |
N,N'-Ethylenethiourea |
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GK2739-250G | Glentham Life Sciences | 250 g | EUR 74.4 |
N,N'-Ethylenethiourea |
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GK2739-25G | Glentham Life Sciences | 25 g | EUR 52.8 |
N,N'-Ethylenethiourea |
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GK2739-500G | Glentham Life Sciences | 500 g | EUR 98.4 |
N,N'-Diphenylthiourea |
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GK5177-25G | Glentham Life Sciences | 25 g | EUR 54 |
N,N-Dimethylglycine |
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GM9748-1G | Glentham Life Sciences | 1 g | EUR 52.8 |
N,N-Dimethylglycine |
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GM9748-25G | Glentham Life Sciences | 25 g | EUR 141.6 |
N,N-Dimethylglycine |
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GM9748-5G | Glentham Life Sciences | 5 g | EUR 69.6 |
N,N'-Dicyclohexylcarbodiimide |
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GE6064-25G | Glentham Life Sciences | 25 g | EUR 56.4 |
N,N'-Dibutylthiourea |
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GE7579-100G | Glentham Life Sciences | 100 g | EUR 67.2 |
N,N'-Dibutylthiourea |
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GE7579-250G | Glentham Life Sciences | 250 g | EUR 98.4 |
N,N'-Dibutylthiourea |
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GE7579-25G | Glentham Life Sciences | 25 g | EUR 54 |
N,N'-Dibutylthiourea |
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GE7579-500G | Glentham Life Sciences | 500 g | EUR 151.2 |
N,N'-Carbonyldiimidazole |
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GE9348-100G | Glentham Life Sciences | 100 g | EUR 98.4 |
N,N'-Carbonyldiimidazole |
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GE9348-1KG | Glentham Life Sciences | 1 kg | EUR 322.8 |
N,N'-Carbonyldiimidazole |
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GE9348-250G | Glentham Life Sciences | 250 g | EUR 151.2 |
N,N'-Carbonyldiimidazole |
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GE9348-25G | Glentham Life Sciences | 25 g | EUR 60 |
N-n-Butylbenzenesulfonamide |
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S-677 | Scientific Laboratory Supplies | 1ML | EUR 84 |
N,N''-Dicyclohexylcarbodiimide |
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GE6064-25 | Glentham Life Sciences | 25 | EUR 16.6 |
N,N''-Dibutylthiourea |
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GE7579-100 | Glentham Life Sciences | 100 | EUR 26.1 |
N,N''-Dibutylthiourea |
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GE7579-25 | Glentham Life Sciences | 25 | EUR 16.4 |
N,N''-Dibutylthiourea |
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GE7579-250 | Glentham Life Sciences | 250 | EUR 51.4 |
N,N''-Dibutylthiourea |
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GE7579-500 | Glentham Life Sciences | 500 | EUR 95 |
N,N''-Carbonyldiimidazole |
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GE9348-1 | Glentham Life Sciences | 1 | EUR 261 |
N,N''-Carbonyldiimidazole |
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GE9348-100 | Glentham Life Sciences | 100 | EUR 51.4 |
N,N''-Carbonyldiimidazole |
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GE9348-25 | Glentham Life Sciences | 25 | EUR 19.8 |
N,N''-Carbonyldiimidazole |
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GE9348-250 | Glentham Life Sciences | 250 | EUR 95 |
N,N-Dimethylglycine |
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GM9748-1 | Glentham Life Sciences | 1 | EUR 14.8 |
N,N-Dimethylglycine |
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GM9748-25 | Glentham Life Sciences | 25 | EUR 87.1 |
N,N-Dimethylglycine |
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GM9748-5 | Glentham Life Sciences | 5 | EUR 27.8 |
N,N-Dimethylethanolamine |
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GK6337-100 | Glentham Life Sciences | 100 | EUR 18.9 |
N,N''-Ethylenethiourea |
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GK2739-1 | Glentham Life Sciences | 1 | EUR 98.8 |
N,N''-Ethylenethiourea |
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GK2739-100 | Glentham Life Sciences | 100 | EUR 27.8 |
N,N''-Ethylenethiourea |
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GK2739-25 | Glentham Life Sciences | 25 | EUR 15.9 |
N,N''-Ethylenethiourea |
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GK2739-500 | Glentham Life Sciences | 500 | EUR 55.4 |
N,N''-Diphenylthiourea |
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GK5177-100 | Glentham Life Sciences | 100 | EUR 22.9 |
N,N''-Diphenylthiourea |
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GK5177-25 | Glentham Life Sciences | 25 | EUR 13.9 |
Buparlisib |
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HY-70063 | MedChemExpress | 100mg | EUR 308.4 |
Buprofezin |
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S-4254 | Scientific Laboratory Supplies | 1ML | EUR 204 |
Buprofezin |
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TBZ2581 | ChemNorm | 250mg | Ask for price |
N,N-Dimethyldodecylamine N-oxide, 98% |
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GC9190-1 | Glentham Life Sciences | 1 | EUR 45.2 |
N,N-Dimethyldodecylamine N-oxide, 98% |
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GC9190-25 | Glentham Life Sciences | 25 | EUR 339 |
N,N-Dimethyldodecylamine N-oxide, 98% |
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GC9190-5 | Glentham Life Sciences | 5 | EUR 113.1 |
Buparvaquone |
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HY-17581 | MedChemExpress | 50mg | EUR 157.2 |
Buparlisib [USAN:INN] |
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20-abx183918 | Abbexa |
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Bupleurotoxin |
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TBZ0120 | ChemNorm | unit | Ask for price |
Buprenorphine (BTG) |
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abx080003-1mg | Abbexa | 1 mg | EUR 543.6 |
Buprenorphine (BSA) |
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abx080004-1mg | Abbexa | 1 mg | EUR 560.4 |
Buprenorphine (BSA) |
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abx080028-1mg | Abbexa | 1 mg | EUR 543.6 |
Hydroxy Bupropion |
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B8305-10 | ApexBio | 10 mg | EUR 308.4 |
Hydroxy Bupropion |
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B8305-5 | ApexBio | 5 mg | EUR 262.8 |